Composite

Part:BBa_K4011018:Design

Designed by: Ziteng Feng   Group: iGEM21_LINKS_China   (2021-10-21)


pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3375
    Illegal BglII site found at 3582
    Illegal BglII site found at 4200
    Illegal XhoI site found at 2426
    Illegal XhoI site found at 4699
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3609
    Illegal AgeI site found at 1987
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2412
    Illegal BsaI site found at 4685
    Illegal BsaI.rc site found at 205
    Illegal BsaI.rc site found at 2662
    Illegal BsaI.rc site found at 4935
    Illegal SapI.rc site found at 4447


Design Notes

1. All codons were optimized for S. cerevisiae based on S. cerevisiae codon bias

2. Homologous recombination of S. cerevisiae was avoided by choosing different promoters and terminators for each expression casette (SaACS2 and AeAT9)


Source

pTEF1, tADH1, pRPL8B, and tSSA1 all come from S. cerevisiae, while SaACS2 comes from Salmonella enterica and AeAT9 comes from Actinidia eriantha

References